Methods for determining L-glutamate in soy sauce with L-glutamate oxidase.
نویسندگان
چکیده
منابع مشابه
Production of D-Glutamate from L-Glutamate with Glutamate Racemase and L-Glutamate Oxidase.
We studied production of D-glutamate from L-glutamate using a bioreactor consisting of two columns of sequentially connected immobilized glutamate racemase (EC 5.1.1.3, from Bacillus subtilis IFO 3336) and L-glutamate oxidase (EC 1.4.3.11, from Streptomyces sp. X119-6): L-glutamate was racemized by the glutamate racemase column, and then L-glutamate was oxidized by the L-glutamate oxidase colum...
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A monosodium glutamate (MSG) biosensor with immobilized L-glutamate oxidase (L-GLOD) has been developed and studied for analysis of MSG in sauces, soup etc. The immobilized enzymatic membrane was attached with oxygen electrode with a push cap system. The detection limit of the sensor was 1 mg/dl and the standard curve was found to be linear upto 20 mg/dl. Response time of the sensor was 2 min. ...
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In the title compound, CH(6)N(3) (+)·C(5)H(8)NO(4) (-), there are two independent cations and two independent anions in the asymmetric unit. In the crystal structure, cations and anions are linked by inter-molecular N-H⋯O hydrogen bonds into a three-dimensional network.
متن کاملArg305 of Streptomyces L-glutamate oxidase plays a crucial role for substrate recognition.
Recently, we have solved the crystal structure of L-glutamate oxidase (LGOX) from Streptomyces sp. X-119-6 (PDB code: 2E1M), the substrate specificity of which is strict toward L-glutamate. By a docking simulation using L-glutamate and structure of LGOX, we selected three residues, Arg305, His312, and Trp564 as candidates of the residues associating with recognition of L-glutamate. The activity...
متن کاملImplantable patch sensor for L-glutamate in hippocampal slices.
We describe an implantable patch sensor for monitoring L-glutamate in hippocampal slices in submerged and interface preparations. This patch sensor is prepared by excising the cell membrane in the CA3 region of a hippocampal slice in a submerged preparation, and then implanted in the target neuronal region (CA1) of mouse hippocampal slices. The lifetime of the sensor was 3-8 min for the interfa...
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ژورنال
عنوان ژورنال: Agricultural and Biological Chemistry
سال: 1984
ISSN: 0002-1369,1881-1280
DOI: 10.1271/bbb1961.48.181